Una mácula es un cambio de color en la piel causada por una alteración en la pigmentación del riego sanguíneo o por salida de sangre. Son cambios de color, tamaño y forma.
Mejoras después del tratamiento con Factores de Crecimiento
Los resultados de nuestro estudio indicaron que el 5% de CGF puede reducir significativamente el daño de los fibroblastos de la piel humana inducida por UVA, mejorar significativamente la viabilidad de los NHDF y disminuir en gran medida el efecto de irradiación UVA (P <0.05). Las tasas de migración del grupo normal y los NHDF irradiados con UVA en el grupo con 5% de CGF tuvieron tasas de migración significativamente mayores (P <0.05), en comparación con el grupo del medio de control. Las tasas de migración de los NHDF irradiados con UVA en 5% de CGF exceden las del grupo normal. Estos resultados mostraron que el 5% de CGF podría promover en gran medida la proliferación celular, la migración y la SOD al mismo tiempo que las cantidades de ROS disminuían notablemente.
Concentrated Growth Factors Can Inhibit Photoaging Damage Induced by Ultraviolet A (UVA) on the Human Dermal Fibroblasts In Vitro
Photoaging is the main cause of extrinsic skin aging. Daily exposure to ultraviolet A (UVA) accelerates the process of photoaging. The present study aimed to understand the role of concentrated growth factors (CGF) on UVA irradiated human skin cells.
We isolated and subcultured normal human dermal fibroblasts (NHDFs) from 6 different human dorsal skins and established photoaging models of NHDFs irradiated by UVA to detect the influence of CGF on fibroblasts in vitro. Three groups were examined: normal, cellular photoaging model (total dosages of 18J·cm−2), and cellular photoaging model plus CGF. In our study, we used the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay method to measure the cell viability. We also used reactive oxygen species (ROS) assay and superoxide dismutase (SOD) assay to measure respectively the amount of oxygen free radicals and antioxidative enzymes. We compared the migration rates among the photoaging model groups, the control groups, and the CGF-treated culture medium groups that were irradiated.
Our study results indicated that 5% CGF can reduce UVA-induced human skin fibroblasts damage significantly, improve the viability of NHDFs significantly, and largely decrease the UVA irradiation effect (P<0.05). The migration rates of the normal group and the UVA-irradiated NHDFs in the 5% CGF group had significantly increased migration rates (P<0.05), compared to the control medium group. The migration rates of the UVA-irradiated NHDFs in 5% CGF exceed those of the normal group. These results showed that 5% CGF could greatly promote cellular proliferation, migration, and SOD at the same time that the amounts of ROS were markedly decreased.
These experimental findings offer some important insights into CGF’s capacity for scavenging ROS, improving SOD, and increasing migration rates in NHDFs irradiated by UVA.
MeSH Keywords: Antioxidants, Reactive Oxygen Species, Skin Diseases, Superoxide Dismutase, Ultraviolet Rays.
Continua la publicación en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540633/
Skin rejuvenation using cosmetic products containing growth factors, cytokines, and matrikines: a review of the literature
Skin aging is primarily due to alterations in the dermal extracellular matrix, especially a decrease in collagen I content, fragmentation of collagen fibrils, and accumulation of amorphous elastin material, also known as elastosis. Growth factors and cytokines are included in several cosmetic products intended for skin rejuvenation because of their ability to promote collagen synthesis. Matrikines and matrikine-like peptides offer the advantage of growth factor-like activities but better skin penetration due to their much smaller molecular size. In this review, we summarize the commercially available products containing growth factors, cytokines, and matrikines for which there is evidence that they promote skin rejuvenation.
Keywords: cosmetics, skin, aging, growth factor, cytokine, matrikine
Skin aging is a natural process caused by both intrinsic changes and extrinsic damage.1,2 Much of the change occurs in the dermis, which is mostly composed of a dense, collagen-rich extracellular matrix (ECM) that provides structure and support for the skin cells and confers tensile strength and firmness to the skin (Figure 1).1,2 Elastic fibers, which are made up of a cross-linked elastin core within fibrillin-based microfibrils, are key secondary components of the dermis that provide elasticity, resilience, and added tensile strength.3 Important changes also occur in the epidermis, most notably the accumulation of corneocytes, which causes the skin to take on a rough and dull appearance.1,4 In addition, reduced skin vasculature and structural changes to the subcutaneous tissue participate in skin aging.
Continua la publicación en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108505/